cell culture human cscc cell line siha  (ATCC)

Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Article Title: Sulfiredoxin may promote metastasis and invasion of cervical squamous cell carcinoma by epithelial-mesenchymal transition.

doi: 10.1177/1010428317695942

Figure Lengend Snippet: Figure 1. Expression of Srx and E-cadherin in cervical squamous cell carcinoma tissues. (a) IHC for the Srx and E-cadherin protein and negative control (×200) in paraffin-embedded sections of normal cervical (NC) tissues, high-grade squamous intraepithelial lesions (HSILs) and cervical squamous cervical cancer (CSCC) tissues. (b) IRS of Srx and E-cadherin expression in NC, HSIL and CSCC tissues. *p < 0.05, **p < 0.05.

Article Snippet: The immunostaining intensity of Srx was assessed as follows: 0 (0) = no staining, 1+ (1–4) = weak staining, 2+ (4–8) = moderate staining and 3+ (8–12) = intense staining.19 Cell lines and cell culture Human CSCC cell lines (SiHa) were obtained from Proteintech and were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, USA), supplemented with 10% fetal bovine serum (Life Technologies, USA) at 37°C in a humidified incubator containing 5% CO2.

Techniques: Expressing, Negative Control

Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Article Title: Sulfiredoxin may promote metastasis and invasion of cervical squamous cell carcinoma by epithelial-mesenchymal transition.

doi: 10.1177/1010428317695942

Figure Lengend Snippet: Figure 3. Srx knockdown efficiency was shown both in mRNA and protein level in HeLa and SiHa cells lines. (a) qRT-PCR analyses showing the expression of Srx gene in different group of HeLa cells (left panel) and SiHa cells (right panel). (b) Western blot analysis showing the expression of Srx in HeLa cells (left panel) and SiHa cells (right panel). The results were expressed as mean ± SD. *p < 0.05.

Article Snippet: The immunostaining intensity of Srx was assessed as follows: 0 (0) = no staining, 1+ (1–4) = weak staining, 2+ (4–8) = moderate staining and 3+ (8–12) = intense staining.19 Cell lines and cell culture Human CSCC cell lines (SiHa) were obtained from Proteintech and were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, USA), supplemented with 10% fetal bovine serum (Life Technologies, USA) at 37°C in a humidified incubator containing 5% CO2.

Techniques: Knockdown, Quantitative RT-PCR, Expressing, Western Blot

Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Article Title: Sulfiredoxin may promote metastasis and invasion of cervical squamous cell carcinoma by epithelial-mesenchymal transition.

doi: 10.1177/1010428317695942

Figure Lengend Snippet: Figure 4. Downregulation of Srx reduces invasion and migration in HeLa and SiHa cells. (a) (upper panels) and (c) (upper panels) Matrigel invasion assay was performed in HeLa and SiHa cells with Srx knockdown. (a) (lower panels) and (c) (lower panels) The effects of Srx downregulation on the migration in HeLa and SiHa cells was assessed by Transwell chamber assay without Matrigel coating. (b) After 24 h of incubation, the number of cell invasion and migration in HeLa cells was counted under the microscope (×120). (d) After 24 h of incubation, the number of invaded cells and migrated cells of SiHa cells was counted under the microscope (×120). The values are expressed as the mean ± SD. *p < 0.05.

Article Snippet: The immunostaining intensity of Srx was assessed as follows: 0 (0) = no staining, 1+ (1–4) = weak staining, 2+ (4–8) = moderate staining and 3+ (8–12) = intense staining.19 Cell lines and cell culture Human CSCC cell lines (SiHa) were obtained from Proteintech and were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, USA), supplemented with 10% fetal bovine serum (Life Technologies, USA) at 37°C in a humidified incubator containing 5% CO2.

Techniques: Migration, Invasion Assay, Knockdown, Transwell Chamber Assay, Incubation, Microscopy

Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Article Title: Sulfiredoxin may promote metastasis and invasion of cervical squamous cell carcinoma by epithelial-mesenchymal transition.

doi: 10.1177/1010428317695942

Figure Lengend Snippet: Figure 5. Srx affected EMT markers and morphology change in SiHa cells. (a) Quantitative RT-PCR showed the expression of EMT-related genes in control, ShNT and ShSrx cells. (b and c) Western blot analysis of the protein levels of Srx, E-cadherin and vimentin in control cells, stable ShNT and ShSrx cells. The results were expressed as mean ± SD (*p < 0.05). (d) The effect of low expression of Srx was examined as cell morphological changes by optical microscopy (×120).

Article Snippet: The immunostaining intensity of Srx was assessed as follows: 0 (0) = no staining, 1+ (1–4) = weak staining, 2+ (4–8) = moderate staining and 3+ (8–12) = intense staining.19 Cell lines and cell culture Human CSCC cell lines (SiHa) were obtained from Proteintech and were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, USA), supplemented with 10% fetal bovine serum (Life Technologies, USA) at 37°C in a humidified incubator containing 5% CO2.

Techniques: Quantitative RT-PCR, Expressing, Control, Western Blot, Microscopy